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Arpita Sharma

2210 Scotties Palace Okland, SN 22012

Email: arpita_82@hotmail.com

Phone: 879-521-3354

Summary:

·         Over 6 years of experience in analytical methods for analysis of drugs, toxicants, steroids and amino acids and proteins.

·         Experience in extraction procedures including liquid-liquid and solid-phase extractions.

·         Over 3 years of experience in the Quality Assurance/Quality Management areas.

·         Experience in interacting with technical staff.

·         Excellent communication and interpersonal skills.

·         Learn new concepts quickly, constantly striving for a new and better way to do things.

·         Dynamic team player able to multitask, high energy level, strong work ethic, committed to results, and adept at working with time deadlines.

Education:

University of St. Thomas Minnesota, USA - Master of Science in Software Systems

University of Kurukshetra, India - Bachelor of Science in Chemistry

Professional Experiences:

Graduate Assistant, University of Minnesota, 2009-present

• Assist in analytical toxicology and sample analysis.
• Analysis of samples using ELISA, HPLC-diode array detector, gas chromatography-mass spectrometry, spectrophotometer.
• Protein separation by electrophoresis

Assistant Scientist, University of Minnesota, July 2004- 2009

• Hands on experience with DNA and RNA extraction from Biological Samples. 
• Experience performing protein purification using immuno-affinity columns.
• Developed and managed large QA/QC program for Minnesota Veterinary Diagnostic Laboratory to qualify for AAVLD accreditation.

Safety Officer July 2005- May 2007

• Perform audits of research lab to ensure that the lab conform to the University of Minnesota safety regulations including functioning of laboratory and safety equipment and proper chemical storage and handling.
• Ensure that researchers are aware of and can access pertinent safety documents and that all training records are current.
• Report your audit findings to appropriate PI/Lab Supervisors, and inform them of their responsibility to take action on any noted deficiencies.

SW Programmer/Tester, University of Minnesota, July 2003 - June 2004

• Worked on Graduation Schedule Coordinator (GSC) web-based application
• Defining website specifications and high level design which include multifaceted security and navigation rules and a variety of user and data flows
• Worked closely with end-users to understand objectives and goals of the website and to ensure their needs are met.
• Extracting information from various legacy databases in different departments, data processing and data presentation to the end user through web browser.

Publications*

Singh AK. Gupta S. Jiang Y. Younus M. Ramzan M. In vitro neurogenesis from neural progenitor cells isolated from the hippocampus region of the brain of adult rats exposed to ethanol during early development through their alcohol-drinking mothers. Alcohol & Alcoholism. 44(2):185-98, 2009.

Dolliver H. Kumar K. Gupta S. Singh A. Application of enzyme-linked immunosorbent assay analysis for determination of monensin in environmental samples. J. Environmental Quality. 37(3):1220-6, 2008 May-Jun.

Singh AK. Gupta S. Jiang Y. Oxidative stress and protein oxidation in the brain of water drinking and alcohol drinking rats administered the HIV envelope protein, gp120. J Neurochemistry. 104(6):1478-93, 2008.

Singh AK. Jiang Y. Gupta S. Effects of chronic alcohol drinking on receptor-binding, internalization, and degradation of human immunodeficiency virus 1 envelope protein gp120 in hepatocytes.

Alcohol. 41(8):591-606, 2007 Dec.

Singh AK. Jiang Y. Gupta S. Benlhabib E. Effects of chronic ethanol drinking on the blood brain barrier and ensuing neuronal toxicity in alcohol-preferring rats subjected to intraperitoneal LPS injection. Alcohol & Alcoholism. 42(5):385-99, 2007 Sep-Oct.

Singh AK. Jiang Y. Benlhabib E. Gupta S. Herbal mixtures consisting of puerarin and either polyenylpho-sphatidylcholine or curcumin provide comprehensive protection against alcohol-related disorders in P rats receiving free choice water and 15% ethanol in pure water. J Med Food. 10:526-42, 2007.

Singh AK. Gupta S. Barnes A. Carlson JM. Ayers JK. Red blood cell erythropoietin, not plasma erythropoietin, concentrations correlate with changes in hematological indices in horses receiving a single dose of recombinant human erythropoietin by subcutaneous injection. J Vet Pharm Ther. 30(2):175-8, 2007 Apr.

Singh AK and Gupta S (2007) Analysis of recombinant human erythropoietin and darbepoietin in spiked plasma. Proteomics-Clin. Appl. 1:626-639.

Gupta S, Sage A, Singh AK Screening and confirmation of recombinant human erythropoietin and darbepoietin-a in spiked plasma samples from drug-free horses. Ana. Chem. Acta. 67:1442-1452, 2005.

Kumar K, Thompson A, Singh AK, Chander Y, Gupta S. Enzyme-linked immunosorbent assay for ultratrace determination of antibiotics in aqueous samples. J Environ Qual. 33:250-256, 2004.

*My key role in these projects includes sample extraction and analysis. I used ELISA, HPLC, mass spectrometry and gas chromatography for these projects.

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