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University of Zurich § Zurich , Switzerland
University of Zurich , Institute
of Biochemistry ▪ Zurich , Switzerland
University of Sofia ▪ Sofia ,
Bulgaria
Technical School ▪ Burgas ,
Bulgaria
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Profile
|
Research scientist currently employed in the
analytical instrumentation manufacturing industry. Professional, with versatile
scientific and technical skills and attention to detail. Possesses in-depth
knowledge of the theoretical principles and the practical applications of calorimetry
and molecular spectroscopy. Expertise in the thermodynamic characterization of proteins
and nucleic acids including analysis of their stability and binding
interactions. Experienced in the use of a wide variety of laboratory analytical
instrumentation, its maintenance and troubleshooting. Dependable and consistent, precise data collection and
evaluation, methods
development, capable of research and interpretation of scientific findings.
Constantly engaged in product
optimization. Fluent in English, native Bulgarian speaker,
functional German and Russian. Proficient in Windows, MS Office, as well as
various scientific data analysis and fitting applications, like Origin and
SigmaPlot.
Areas of Expertise
|
Isothermal Titration Calorimetry
Differential Scanning Calorimetry
UV/VIS/NIR,
Fluorescence, and CD Spectroscopy
Stopped-Flow Kinetic Methods
Spectroscopic Hardware and Software
Optimization
Spectroscopic
Instruments Installation and Repair
Bench Chemistry
Protein Expression and Purification
Molecular Biology
Data Analysis and Fitting
Laboratory Personnel Training
College Level Teaching – Biochemistry Lab
Course
Professional Experience
|
On-Line Instrument
Systems, Inc. (OLIS, Inc.) § Bogart , GA
Æ Staff Scientist Jun 2006-Present
Collaborates in the design and optimization
of custom-made instruments with engineers, software specialists, and clients. In-house
and on-site calibration and testing of components and final product. Adjusts
software parameters for existing hardware configuration and conducts optical
bench optimization. Complies with benchmark requirements for instrument
configuration; calibrates monochromator wavelength, documents standard spectra;
analyzes and optimizes the light throughput, monitors overall system performance,
resolves and communicates software and hardware related issues, creates a certificate
of calibration. Coordinates the tasks of programmers, mechanical and electronic
technicians at the final stage of instrument validation to meet delivery
deadlines. Verifies the fitting algorithms and recommends improvements; conducts
literature research related to ongoing projects.
4 Immediately
identifies and solves technical issues through e-mail, telephone, and remote PC
access;
4 Supports
the customer in obtaining the best results by consulting about scientific
issues, such as proper sample preparation, fitting, detection limits and
instrument parameters adjustments;
4 On-site
installation of the instrument -including
unpacking, assembling and testing of the instrument;
4 Designs and
conducts training for the laboratory personnel -hardware and software
introduction, short theory of the principles of spectrophotometer operation as
well as hands-on training using the customers materials;
4 Writes
an installation/repair report outlying resolved issues for later reference and
unresolved issues with the necessary follow up;
4 Communicates
customers demands and wishes, and works with the management to meet them;
4 Independently
arranges travel, including flight, hotel, and rental car booking; occasionally transportation
and unloading of the instrument;
4 Attends
science conferences and trade shows;
Research Experience
|
Æ Postdoctoral Research Associate Oct 2004 - May 2006
Æ Ph.D
Student Jan
2001 – Sep 2004
4 Preparation
of samples including oligonucleotid design, DNA mutagenesis, bacterial cells
transformation, cell growth, protein expression, isolation and purification;
4 Calorimetric
and spectroscopic measurement of the binding and stability of the protein and
DNA. Analysis of the obtained data to determine the thermodynamic parameters
(free energy, enthalpy, entropy and heat capacity change) of the binding and
unfolding reactions;
4 Publication
of the results in collaboration with crystallographers and computational
chemists;
4 Anaerobic
purification and investigation of the function of bacterial Complex I as a
sodium pump;
4 Study of
the charge–dependent properties of bacterial b - Lactamase using fluorescence quenching;
Notable Accomplishments:
r Recognized by the management for superior
installations, which resulted in the lowest technical or customer issues
r Increased the sensitivity of our line of fluorimeters
by 300%
r Implemented novel techniques, such as
fluorescence polarization measurements without the need of moving polarizers
and G-factor correction, circularly polarized luminescence (CPL), and fluorescence-detected
circular dichroism (FDCD) using gated photon counting detector
r Designed, coordinated, and conducted 70+
installation/training sessions in more than 10 countries; continually
acknowledged by supervisors regarding the exclusively positive feedback received
from clients
r Successfully completed highly complicated installations
– among them: the first CD instrument in the world using synchrotron beamline
as a light source (Oxfordshire UK); novel laser flash photolysis system
utilizing fast shutter attached to a rapid scanning spectrophotometer (Pisa,
Italy); the first commercial CPL (circularly polarized luminescence) instrument
in the world (New Orleans, LA)
r On-site hardware conversion and software
upgrade of old Aminco spectrophotometers (Oulu ,Finland and Frankfurt , Germany
r Superior time management skills – efficiently
completes tasks like testing of outgoing instruments or installations on
schedule
r Completion of a Ph.D. project in less than
4 years resulting in 3 first author publications in peer-reviewed scientific
journals and a book chapter
Education
|
Doctor of
Philosophy in Biochemistry
Dissertation: Biophysical Characterization of the Site-Specific
Binding of Integrase TN916 to its Cognate DNA
Master of Science
in Molecular Biology
Diploma Thesis: Charge-Dependent Properties of β-Lactamase I from Bacillus cereus
Diploma in Industrial
Electrotechnics
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